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 Alcohol Strip Test:
 
      
 
 INTRODUCTION
 
 

The One Step Saliva Alcohol Screening Test is a simple, accurate and convenient chemical assay for the rapid and qualitative detection of human blood alcohol levels. The one step saliva alcohol screening test is fast and easy to use. The results are read visually without the need for instrumentation. It shows a high degree of sensitivity and can detect up to 0.02% alcohol concentration in saliva.

Note:  This test shall not be used to determine one's ability to legally operate a motor vehicle or heavy equipment. Any decision based on the results of this test will be the sole responsibility of the user.

 
 
           
 
PRINCIPLE OF PROCEDURE
 

The strips are disposable plastic strips which consist of a single reagent pad. The test is designed to provide a qualitative measurement of alcohol in a saliva specimen by comparing the color developed at the end of the reaction period with the color chart provided. The test strips employ alcohol oxidase, peroxidase and a hydrogen donor indicator in a carrier matrix supported on the strip with the alcohol oxidase functioning as a catalyst to convert any ethanol present along with ambient oxygen to acetaldehyde and hydrogen peroxide. The peroxidase functions as catalyst to induce a color change in the hydrogen donor and convert the hydrogen peroxide to water.

The reactions are as follows:

ETOH + O2 ¡┣ CH2CHO

H2O2  + TMB. 2OH ¡┣ Colored substrate + 2H2O