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The strips are disposable
plastic strips which consist of a single reagent pad.
The test is designed to provide a qualitative
measurement of alcohol in a saliva specimen by comparing
the color developed at the end of the reaction period
with the color chart provided. The test strips employ
alcohol oxidase, peroxidase and a hydrogen donor
indicator in a carrier matrix supported on the strip
with the alcohol oxidase functioning as a catalyst to
convert any ethanol present along with ambient oxygen to
acetaldehyde and hydrogen peroxide. The peroxidase
functions as catalyst to induce a color change in the
hydrogen donor and convert the hydrogen peroxide to
water.
The reactions are as
follows:
ETOH + O2 ¡┣ CH2CHO
H2O2 + TMB. 2OH ¡┣
Colored substrate + 2H2O |